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N Use By Plants
Nitrate Assimilation
Ammonia Assimilation
Glu, Gln, Asn, Gly, Ser
Aminotransferases
Asp, Ala, GABA
Val, Leu, Ileu, Thr, Lys
Pro, Arg, Orn
Polyamines
Non-protein AAs
Alkaloids
Sulfate Assimilation
Cys, Met, AdoMet, ACC
His, Phe, Tyr, Tryp
Secondary Products
Onium Compounds
Enzymes
Methods
Simulation
References
HORT640 - Metabolic Plant Physiology

References, dhurrin

Bak S, Kahn RA, Nielsen HL, Moller BL, Halkier BA. Cloning of three A-type cytochromes P450, CYP71E1, CYP98, and CYP99 from Sorghum bicolor (L.) Moench by a PCR approach and identification by expression in Escherichia coli of CYP71E1 as a multifunctional cytochrome P450 … Plant Mol. Biol. 36: 393-405 (1998).

Bak S, Nielsen HL, Halkier BA. The presence of CYP79 homologues in glucosinolate-producing plants shows evolutionary conservation of the enzymes in the conversion of amino acid to aldoxime in the biosynthesis of cyanogenic glucosides and glucosinolates. Plant Mol. Biol. 38: 725-734 (1998).

Bak S, Olsen CE, Halkier BA, Moller BL. Transgenic tobacco and Arabidopsis plants expressing the two multifunctional sorghum cytochrome P450 enzymes, CYP79A1 and CYP71E1, are cyanogenic and accumulate metabolites derived from intermediates in dhurrin biosynthesis. Plant Physiol. 123: 1437-1448 (2000).

Bak S, Olsen CE, Petersen BL, Moller BL, Halkier BA. Metabolic engineering of p-hydroxybenzylglucosinolate in Arabidopsis by expression of the cyanogenic CYP79A1 from Sorghum bicolor. Plant J. 20: 663-671 (1999).

Busk PK, Moller BL. Dhurrin synthesis in sorghum is regulated at the transcriptional level and induced by nitrogen fertilization in older plants. Plant Physiol. 129: 1222-1231 (2002).

Celenza JL. Metabolism of tyrosine and tryptophan - new genes for old pathways. Curr. Opin. Plant Biol. 4: 234-240 (2001).

Cicek M, Esen A. Structure and expression of a dhurrinase (beta-glucosidase) from sorghum. Plant Physiol. 116: 1469-1478 (1998).

Coneva V, Zhu T, Colasanti J. Expression differences between normal and indeterminate1 maize suggest downstream targets of ID1, a floral transition regulator in maize. J. Exp. Bot. 58: 3679-3693 (2007).

Haskins FA, Gorz HJ. Relationship between contents of leucoanthocyanidin and dhurrin in sorghum leaves. Theor. Appl. Genet. 73: 2-3 (1986).

Haskins FA, Gorz HJ. Independent inheritance of genes for dhurrin and leucoanthocyanidin in a sorghum cross. Crop Sci. 28: 864-865 (1988).

Jones PR, Moller BL, Hoj PB. The UDP-glucose:p-hydroxymandelonitrile-O-glucosyltransferase that catalyzes the last step in synthesis of the cyanogenic glucoside dhurrin in Sorghum bicolor. Isolation, cloning, heterologous expression, and substrate specificity. J. Biol. Chem. 274: 35483-35491 (1999).

Kahn RA, Bak S, Svendsen I, Halkier BA, Moller BL. Isolation and reconstitution of cytochrome P450ox and in vitro reconstitution of the entire biosynthetic pathway of the cyanogenic glucoside dhurrin from sorghum. Plant Physiol. 115: 1661-1670 (1997).

Kristensen C, Morant M, Olsen CE, Ekstrom CT, Galbraith DW, Moller BL, Bak S. Metabolic engineering of dhurrin in transgenic Arabidopsis plants with marginal inadvertent effects on the metabolome and transcriptome. Proc. Natl. Acad. Sci. U.S.A. 102: 1779-1784 (2005).

MacFarlane IJ, Lees EM, Conn EE. The in vitro biosynthesis of dhurrin, the cyanogenic glycoside of Sorghum bicolor. J. Biol. Chem. 250: 4708-4713 (1975).

Memelink J. Tailoring the plant metabolome without a loose stitch. Trends Plant Sci. 10: 305-307 (2005).

Nielsen KA, Hrmova M, Nielsen JN, Forslund K, Ebert S, Olsen CE, Fincher GB, Moller BL. Reconstitution of cyanogenesis in barley (Hordeum vulgare L.) and its implications for resistance against the barley powdery mildew fungus. Planta 223: 1010-1023 (2006).

Petersen BL, Andreasson E, Bak S, Agerbirk N, Halkier BA. Characterization of transgenic Arabidopsis thaliana with metabolically engineered high levels of p-hydroxybenzylglucosinolate. Planta 212: 612-618 (2001).

Piotrowski M. Primary or secondary? Versatile nitrilases in plant metabolism. Phytochemistry 69: 2655-2667 (2008).

Tattersall DB, Bak S, Jones PR, Olsen CE, Nielsen JK, Hansen ML, Hoj PB, Moller BL. Resistance to an herbivore through engineered cyanogenic glucoside synthesis. Science 293: 1826-1828 (2001).

Thorsoe KS, Bak S, Olsen CE, Imberty A, Breton C, Moller BL. Determination of catalytic key amino acids and UDP sugar donor specificity of the cyanohydrin glycosyltransferase UGT85B1 from Sorghum bicolor. Molecular modeling substantiated by site-specific mutagenesis and biochemical analyses. Plant Physiol. 139: 664-673 (2005).

Winkel BS. Metabolic channeling in plants. Annu. Rev. Plant Biol. 55: 85-107 (2004).

Number of references = 21

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