Figure 1 shows a total ion chromatogram of a standard mixture of amino acids (each at 2.5 nmol/uL) derivatized as HFBI derivatives and analyzed by electron ionization (EI) GC-MS using a Finnigan GCQ tandem mass spectrometer equipped with a db1 capillary column (oven temperature program = 100^oC (4 min) to 280^oC at 12^oC per min), scanning over the mass range 210 to 370 amu; a range which detects most of the major electron ionization fragment ions of HFBI-amino acid derivatives.

Structures of various HFBI-amino acid derivatives (listed in order of their retention times) and their chemical ionization (CI) mass spectra (mass range scanned = 200 - 600 amu) and/or electron ionization (EI) mass spectra (mass range scanned = 210 - 370 amu) can be accessed from the following page:

This includes an interactive JavaScript application for calculating the molecular weights, expected molecular ion M+H⁺, and expected fragment ions of the HFBI-amino acid derivatives. This application will function with both Internet Explorer and Netscape browsers.