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HORT640 - Metabolic Plant Physiology
Enzyme Kinetics
The following are links to Java Applets that demonstrate some basic principles of analysis of enzyme kinetics. Data (substrate concentrations and reaction velocities) used in these examples are hypothetical, but can be replaced with real experimental results to caculate Vmax and Km values.
- Single substrate, uncompetitive inhibition, Lineweaver-Burk plot
- Single substrate, uncompetitive inhibition, Eadie-Hofstee plot
- Single substrate, competitive inhibition, Lineweaver-Burk plot
- Single substrate, competitive inhibition, Eadie-Hofstee plot
- Single substrate, noncompetitive inhibition, Lineweaver-Burk plot
- Single substrate, noncompetitive inhibition, Eadie-Hofstee plot
- Two substrates (A and B), kinetics for substrate A, Lineweaver-Burk plot
- Two substrates (A and B), kinetics for substrate B, Lineweaver-Burk plot
- Two substrates (A and B), kinetics for substrate A, Eadie-Hofstee plot
- Two substrates (A and B), kinetics for substrate B, Eadie-Hofstee plot
The following are on-line quizzes that test knowledge of enzyme kinetics using interactive Java Applets:
- Single substrate kinetics with unknown mode of inhibition analyzed using Lineweaver-Burk plots - find Kms, Vmaxs and mode of inhibition [competitive, noncompetitive, or uncompetitive]? - example 1
- Single substrate kinetics with unknown mode of inhibition analyzed using Lineweaver-Burk plots - find Kms, Vmaxs and mode of inhibition [competitive, noncompetitive, or uncompetitive]? - example 2
- Single substrate kinetics with unknown mode of inhibition analyzed using Lineweaver-Burk plots - find Kms, Vmaxs and mode of inhibition [competitive, noncompetitive, or uncompetitive]? - example 3
- Single substrate kinetics with unknown mode of inhibition analyzed using Eadie-Hofstee plots - find Kms, Vmaxs and mode of inhibition [competitive, noncompetitive, or uncompetitive]? - example 4
- Single substrate kinetics with unknown mode of inhibition analyzed using Eadie-Hofstee plots - find Kms, Vmaxs and mode of inhibition [competitive, noncompetitive, or uncompetitive]? - example 5
- Single substrate kinetics with unknown mode of inhibition analyzed using Eadie-Hofstee plots - find Kms, Vmaxs and mode of inhibition [competitive, noncompetitive, or uncompetitive]? - example 6
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David Rhodes
Department of Horticulture & Landscape Architecture
Horticulture Building
625 Agriculture Mall Drive
Purdue University
West Lafayette, IN 47907-2010
Last Update: 01/07/08
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