Murphy / Peer lab

Help!

Sometimes you get to publish all the nitty gritty details in the methods section, and sometimes you don’t. Here are the methods with those details.

Everything that you always wanted to know about auxin transport, but were afraid to ask.

The embryos are easily killed in lines with transparent testa and other light colored seed coats. Here is a gentle method that eliminates fungi without killing the embryo.

Extreme microsomes. Just like Maverick’s.

Total microsomes. Totally cool, dude.

Whole mount immunolocalisation made easy.

Auxin transport

Microsomal prep

DRM prep

Seed surface sterilisation protocol

Immunolocalisation

Protocols

Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.