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Welcome to the "101 Ways" Arabidopsis growth advice website. This site, created for researchers by researchers, reports the results of an applied horticultural science experiment to determine methods of growing Arabidopsis thaliana that are successful, repeatable, and scalable. Below, you can find the top plant growth questions and photographs describing results related to those questions. For more in-depth information, please see the 'Technical Information' section below. A printable, single-page summary of recommendations is provided, as well as technical analyses of irrigation solutions and root media.

Advice by Question:

Click the "D" link preceeding each entry to get details about the answer.
Click the "P" link to see photographs of the experimental results.
* indicates multiple sections are available for that particular experiment.

[D]	[P]	Should a greenhouse, light shelf or growth chamber be used? *
[D]	[P]	What pot size worked the best in this study?
[D]	[P]	What soil mix worked best in this study? *
[D]	[P]	Can a soilless mix be augmented to improve growth?
[D]	[P]	What root media worked best to cleanly remove roots?
[D]	[P]	Does soil need to be pressed down prior to planting?
[D]	[P]	Do seeds need to be misted to germinate?
[D]	[P]	What did this study show about the benefit of draining trays?*
[D]	[P]	What if water HAS to be left in the trays?
[D]	[P]	Did use of capillary matting for sub-irrigation improve growth?
[D]	[P]	Was fertilizer required?
[D]	[P]	What frequency of fertilizing worked best in this study?
[D]	[P]	What fertilizer strength worked best in this study?
[D]	[P]	Did use of slow-release fertilizer result in healthy plants?
[D]	[P]	What light intensity worked best in this study?
[D]	[P]	Can high intensity discharge lights be used?
[D]	[P]	What photoperiod (daylength) worked best in this study?
[D]	[P]	Does growth under 24-hour light hasten production?
[D]	[P]	Does 24-hour illumination damage plants?
[D]	[P]	Can plants be transferred from a low light environment to high light?
[D]	[P]	Can early flowering during long, hot days be avoided?
[D]	[P]	Can a greenhouse table be modified with air-conditioning?
[D]	[P]	Can a shelf unit be modified with air-conditioning?
[D]	[P]	Did any treatments reduce fungus gnat infestation?
[D]	[P]	How much imidacloprid (Marathon 1G) need be applied?
[D]	[P]	Do any insecticides or fungicides burn foliage?
[D]	[P]	I've run out of space in my facility- how can I optimize it?
[D]	[P]	What is my worst Arabidopsis nightmare?
[D]		Is reverse-osmosis purified water required to irrigate the plants?
[D]		What about germination treatments and hydroponic techniques that this study did not examine?

Related References

*Titles have been summarized
*Full References also available: [.pdf]

• The Arabidopsis Information Resource (TAIR)
• ABRC webpage: Handling Arabidopsis Plants and Seeds
• Nottingham Arabidopsis Stock Centre
• Arabidopsis: A Laboratory Manual
• Influence of temp. and imbibition on germination
• Arabidopsis response to short photoperiods
• A hydroponic method for culturing populations of Arabidopsis
• Maximal biomass of Arabidopsis using hydroponics
• Increased vigor through prolongation of the vegetative stage
• Large scale soil culture of Arabidopsis
• The effect of cold treatment on germination and flowering
• A novel high efficiency, low maintenance, hydroponic system

Technical Information

• [.pdf] Printable, Single-page of Recommendations--UPDATED
• [.pdf] Materials & Methods--UPDATED
• [.pdf] Literary References
• HRGH/WSLR Soil Mix Spec Sheets
• [.pdf] Treatment List

HGRH / WSLR Technical Analyses

• [.pdf] Solution Analysis: WSLR 50 ppm N Fertilizer
• [.pdf] Solution Analysis: WSLR 200 ppm N Fertilizer
• [.pdf] Solution Analysis: WSLR Clear Water (tap)
• [.pdf] Solution Analysis: WSLR RO Water
• [.pdf] Solution Analysis: HGRH siphon 200 ppm N Fertilizer
• [.pdf] Solution Analysis: HGRH Fertilizer Water
• [.pdf] Solution Analysis: HGRH RO Water
• [.pdf] Solution Analysis: HGRH Clear Water (tap)
• [.pdf] Media Analysis: MetroMix 360
• [.pdf] Media Analysis: Promix BX
• [.pdf] Media Analysis: Ecolite
• [.pdf] Media Analysis: Promix HP
• [.pdf] Media Analysis: Promix PGX
• [.pdf] Media Analysis: Profile
• [.pdf] Media Analysis: Sunshine Mix LA4
• [.pdf] Media Analysis: Sunshine Mix pH analysis
• [.pdf] Media Analysis: Greenhouse Soil pH
• [.pdf] Media Analysis: Redi-Earth
• [.pdf] Media Analysis: Turface
• [.pdf] Media Analysis: Vermiculite