culms showing waxy bloomWildtype

Member lines: P898012, P954035

Gross epicuticular wax phenotype: Wildtypescanning electron micrograph of abaxial leaf sheath

Epicuticular wax morphology:
(see Jenks et al., 1992 and scanning electron micrograph to the right of this table)

 tissue

 cork-silica cell associated wax

 cuticle wax

 leaf sheath (abaxial surface)

 long filaments

 plates

 leaf blade (abaxial surface)

 short filaments

 thin plates

Linkage group: not applicable

Genotype: BmBmHH

Epicuticular wax load:
(data from Agronomy Farm trial 1992)

 line

 abaxial leaf sheath

 leaf blade

 P898012

 2.24 +/- 0.31

 1.29 +/- 0.13

 P954035

 3.39 +/- 0.41

 1.06 +/- 0.13

Leaf sheath epicuticular wax chemistry: Thin layer chromatogram shoing major wax lipid classes on leaf sheaths

 Leaf sheath epicuticular wax free fatty acids

Gas chromatogram of free fatty acids as methyl ester derivatives
 FFA homologue  % total FFAs  load (mg/cm2)
C16 0.30 0.06 
 C18  0.20   0.04 
C20 0.03  0.01  
C22 0.13   0.03  
C24 0.25  0.06  
C26 0.34  0.08  
C28 74.6   19.7  
C30 24.0   6.15  
C32 0.16  0.04  
 total

100  

26.18 
Wild type sheath wax is composed mainly (90%) of C28 and C30 free fatty acids. Other wax lipids are shown in the TLC at right. Unlabelled spots are unidentified lipids.

Agronomic characters: P898012 mature grain headP954035 mature grain headP954035 mature grain head

 line

 yield without drought (kg/ha)

yield in drought (kg/ha)

 P898012

 6102

 

 P954035

 6204

 1382

P898012 (right) is a Federita type and has less visible wax than P954035 on its culm.

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