Class 12 Mutants

Member lines: bm-4A, bm-7, bm-38

Gross epicuticular wax phenotype: Bloomless

Epicuticular wax morphology:

(see Jenks et al., 1992 and scanning electron micrograph to the right of this table)

tissue	cork-silica cell associated wax	cuticle wax
leaf sheath (abaxial surface)	none	plates
leaf blade (abaxial surface)	none	thin plates

Linkage group: bm3 (bm-4A), bm8 (bm-7) and bm11 (bm-38)

Genotype: bm3bm3HH (bm-4A), bm8bm8HH (bm-7) and bm11bm11HH (bm-38)

Epicuticular wax load:

(data from Agronomy Farm trial 1992)

line	abaxial leaf sheath	leaf blade
bm-4A	0.86 +/- 0.19	1.00 +/- 0.10
bm-7	1.22 +/- 0.16	1.22 +/- 0.08
bm-38	0.99 +/- 0.18	1.09 +/- 0.10

Leaf sheath epicuticular wax chemistry:

Leaf sheath epicuticular wax free fatty acids

FFA homologue	% total FFAs	load (mg/cm2)
C16	6.45	0.52
C18	5.31	0.43
C20	0.29	0.02
C22	0.45	0.04
C24	0.47	0.04
C26	0.36	0.03
C28	58.8	4.97
C30	27.2	2.25
C32	0.67	0.05
total	100	8.35

Class 12 mutant sheath wax contains an average of 80% free fatty acids (FFA).

Agronomic characters:

Bm-38 plants are short, chlorotic, form no basal tillers and are later maturing than most lines in the P954035 background.

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