culms showing waxy bloomClass 11 Mutants

Member lines: bm-24

Gross epicuticular wax phenotype: Sparse bloomscanning electron micrograph of abaxial leaf sheath

Epicuticular wax morphology:
(see Jenks et al., 1992 and scanning electron micrograph to the right of this table)

 tissue

 cork-silica cell associated wax

 cuticle wax

 leaf sheath (abaxial surface)

amorphous globs

smooth

 leaf blade (abaxial surface)

small globs

smooth

Linkage group: h12

Genotype: BmBmh12h12

Epicuticular wax load:
(data from Agronomy Farm trial 1992)

 line

 abaxial leaf sheath

 leaf blade

 bm-24

1.08 +/- 0.31

 1.18 +/- 0.10

Leaf sheath epicuticular wax chemistry: Thin layer chromatogram shoing major wax lipid classes on leaf sheaths

 Leaf sheath epicuticular wax free fatty acids

Gas chromatogram of free fatty acids as methyl ester derivatives
 FFA homologue  % total FFAs  load (mg/cm2)
C16 2.09 0.18
C18  2.18  0.19
C20 0.22 0.02
C22 0.99 0.09
C24 1.24 0.11
C26 0.50 0.04
C28 69.7 6.16
C30 22.6 1.99
C32 0.49 0.04
 total

100

8.83
Bm-24 sheath wax contains 82% free fatty acids (FFA).

Agronomic characters:

Bm-24 forms no basal tillers and has wrinkled leaves.

 

Return to Catalog Index