Class 8 Mutants

Member lines: bm-13

Gross epicuticular wax phenotype: Bloomless scanning electron micrograph of abaxial leaf sheath

Epicuticular wax morphology:
(see Jenks et al., 1992 and scanning electron micrograph to the right of this table)

tissue

cork-silica cell associated wax

cuticle wax

leaf sheath (abaxial surface)

globular filaments

sparse plates

leaf blade (abaxial surface)

small globs

sparse plates

Linkage group: not included in allelism tests

(see Jenks et al., 1992 and scanning electron micrograph to the right of this table)
tissue	cork-silica cell associated wax	cuticle wax
leaf sheath (abaxial surface)	globular filaments	sparse plates
leaf blade (abaxial surface)	small globs	sparse plates

Genotype: bm?bm?HH

Epicuticular wax load: no data

Leaf sheath epicuticular wax chemistry: Thin layer chromatogram shoing major wax lipid classes on leaf sheaths

Leaf sheath epicuticular wax free fatty acids

Gas chromatogram of free fatty acids as methyl ester derivatives

FFA homologue % total FFAs load (mg/cm2)

C16 0.17
no

data

C18 0.13

C20 0.09

C22 0.27

C24 0.49

C26 1.05

C28 79.2

C30 18.3

C32 0.31

total
100

Bm-13 sheath epicuticular wax contains 86% free fatty acids.

Agronomic characters:

Bm-13 was dropped from field trials because mutant plants are weak and stunted with wrinkled leaves.

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FFA homologue	% total FFAs	load (mg/cm2)
C16	0.17	no data
C18	0.13
C20	0.09
C22	0.27
C24	0.49
C26	1.05
C28	79.2
C30	18.3
C32	0.31
total	100