Class 7 Mutants

Member lines: bm-12

Gross epicuticular wax phenotype: Sparse bloomscanning electron micrograph of abaxial leaf sheath

Epicuticular wax morphology:
(see Jenks et al., 1992 and scanning electron micrograph to the right of this table)

 tissue

 cork-silica cell associated wax

 cuticle wax

 leaf sheath (abaxial surface)

 amorphous filaments

 plates

 leaf blade (abaxial surface)

 amorphous filaments

 thin plates

Linkage group: not included in allelism tests

Genotype: BmBmh?h?

Epicuticular wax load: no data

Leaf sheath epicuticular wax chemistry: Thin layer chromatogram shoing major wax lipid classes on leaf sheaths

 Leaf sheath epicuticular wax free fatty acids

Gas chromatogram of free fatty acids as methyl ester derivatives
 FFA homologue  % total FFAs  load (mg/cm2)
C16 0.17

no

data

 C18  0.13
C20 0.09
C22 0.31
C24 0.48
C26 0.44
C28 65.7
C30 31.9
C32 0.61
 total

100

Bm-12 sheath epicuticular wax contains 85% free fatty acids.

culms showing waxy bloomAgronomic characters:

Bm-12 was dropped from field trials because its visual phenotype is difficult to distinguish from the wildtype.

 

 

 

 

 

 

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