Class 6 Mutants

Member lines: bm-29

Gross epicuticular wax phenotype: Sparse bloom scanning electron micrograph of abaxial leaf sheath

Epicuticular wax morphology:
(see Jenks et al., 1992 and scanning electron micrograph to the right of this table)

tissue

cork-silica cell associated wax

cuticle wax

leaf sheath (abaxial surface)

short filaments

plates

leaf blade (abaxial surface)

small globs

sparse plates

Linkage group: not included in allelism tests

(see Jenks et al., 1992 and scanning electron micrograph to the right of this table)
tissue	cork-silica cell associated wax	cuticle wax
leaf sheath (abaxial surface)	short filaments	plates
leaf blade (abaxial surface)	small globs	sparse plates

Genotype: BmBmh?h?

Epicuticular wax load: no data

Leaf sheath epicuticular wax chemistry: Thin layer chromatogram shoing major wax lipid classes on leaf sheaths

Leaf sheath epicuticular wax free fatty acids

Gas chromatogram of free fatty acids as methyl ester derivatives

FFA homologue % total FFAs load (mg/cm2)

C16 0.12
no

data

C18 0.06

C20 0.03

C22 2.58

C24 7.82

C26 18.3

C28 47.2

C30 23.2

C32 0.71

total
100

Bm-29 sheath epicuticular wax contains 82% free fatty acids.

culms showing waxy bloom Agronomic characters:

Bm-29 was dropped from field trials because its visual phenotype is difficult to distinguish from the wildtype.

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FFA homologue	% total FFAs	load (mg/cm2)
C16	0.12	no data
C18	0.06
C20	0.03
C22	2.58
C24	7.82
C26	18.3
C28	47.2
C30	23.2
C32	0.71
total	100