Definitions

A1 = Isotope abundance of precursor during pulse (atom %)

A2 = Isotope abundance of precursor during chase (atom %)

TS = Total time of the simulation (min)

TC = Chase start time (min)

B1 = Initial isotope abundance of metabolic pool of B (atom %)

B2 = Initial pool size of metabolic pool of B (nmol.gfw^-1)

B3 = Rate of synthesis of metabolic pool of B (nmol.min^-1.gfw^-1)

B4 = Combined rate of utilization of metabolic pool of B (nmol.min^-1.gfw^-1)

B5 = Initial isotope abundance of storage pool of B (atom %)

B6 = Initial pool size of storage pool of B (nmol.gfw^-1)

B7 = Rate of transport of metabolic pool of B to storage pool of B (nmol.min^-1.gfw^-1)

B8 = Rate of transport of storage pool of B to metabolic pool of B (nmol.min^-1.gfw^-1)

C1 = Initial isotope abundance of metabolic pool of C (atom %)

C2 = Initial pool size of metabolic pool of C (nmol.gfw^-1)

C3 = Rate of synthesis of metabolic pool of C (nmol.min^-1.gfw^-1)

C4 = Combined rate of utilization of metabolic pool of C (nmol.min^-1.gfw^-1)

C5 = Initial isotope abundance of storage pool of C (atom %)

C6 = Initial pool size of storage pool of C (nmol.gfw^-1)

C7 = Rate of transport of metabolic pool of C to storage pool of C (nmol.min^-1.gfw^-1)

C8 = Rate of transport of storage pool of C to metabolic pool of C (nmol.min^-1.gfw^-1)

D1 = Initial isotope abundance of metabolic pool of D (atom %)

D2 = Initial pool size of metabolic pool of D (nmol.gfw^-1)

D3 = Rate of synthesis of metabolic pool of D (nmol.min^-1.gfw^-1)

D4 = Combined rate of utilization of metabolic pool of D (nmol.min^-1.gfw^-1)

D5 = Initial isotope abundance of storage pool of D (atom %)

D6 = Initial pool size of storage pool of D (nmol.gfw^-1)

D7 = Rate of transport of metabolic pool of D to storage pool of D (nmol.min^-1.gfw^-1)

D8 = Rate of transport of storage pool of D to metabolic pool of D (nmol.min^-1.gfw^-1)

gfw = g fresh weight